منابع مشابه
Colchicine induced embryogenesis and doubled haploid production in maize (Zea mays L.) anther culture
This study involves in vitro androgenesis of <sp...
متن کاملHighly Efficient Doubled-Haploid Production in Wheat (Triticum aestivum L.) via Induced Microspore Embryogenesis
haploid plant per floret (Devaux, 1988). Androgenesis induction in microspores may be affected by various An efficient doubled-haploid production technology that induces factors which cause low induction efficiency and by gehomozygosity can greatly reduce the time and cost of cultivar developnotype dependence (Dunwell, 1985). Most advances toment. Low efficiency of doubled-haploid production pr...
متن کاملEfficient Doubled Haploid Production in Brassica napus via Microspore Colchicine Treatment in vitro and Ploidy Determination by Flow Cytometry
An efficient microspore culture protocol was used to rapidly produce homozygous doubled haploid (DH) progeny of crosses between low erucic canola and high erucic resynthesized rapeseed (Brassica napus L.). Microspores of Canadian cultivars ‘Excel’ and ‘Profit’ as well as three F1 hybrids with the resynthetic line RS239 were treated with colchicine immediately after isolation. Flow cytometry was...
متن کاملHaploid and Doubled Haploid Techniques in Perennial Ryegrass (Lolium perenne L.) to Advance Research and Breeding
The importance of haploid and doubled haploid (DH) techniques for basic and applied research, as well as to improve the speed of genetic gain when applied in breeding programs, cannot be overstated. They have become routine tools in several major crop species, such as maize (Zea mays L.), wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.). DH techniques in perennial ryegrass (Lolium ...
متن کاملProduction of doubled haploid plants of carnation (Dianthus caryophyllus L.) by pseudofertilized ovule culture
Doubled haploids of carnation (Dianthus caryophyllus L.) were obtained by pseudofertilized ovule culture. Emasculated ̄ower buds of carnation were pollinated with pollen inactivated by Xray irradiation. After 2±3 weeks, the ovaries were explanted and were cultured on solid MS medium containing 2 mM NAA, 2 mM BAP and 6% sucrose. Regenerated plants(R0) were morphologically different from the moth...
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ژورنال
عنوان ژورنال: Vavilov Journal of Genetics and Breeding
سال: 2015
ISSN: 1814-5558,1814-554X
DOI: 10.18699/vj15.014